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Preparation of cell culture media containing Zn 2+ , Cu 2+ , SiO 3 2 , Qu or their combinations Cell experiments were performed using the media containing a certain concentration of Zn 2+ , Cu 2+ , SiO 3 2 , Qu or their combinations, which were prepared by adding the corresponding amount of zinc chloride, copper chloride, sodium silicate, Qu or their combinations in normal cell culture media, respectively