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The dose needed depends on how severe the deficiency is and what each person needs

Normally sequestered in cytoplasm by Keap1

However, this mechanism requires the presence of susceptible substrates

Because IGFBP-3 also carries IGF-1, when IGF-1 is administered on its own, the carrier IGFBP-3 saturates with IGF-1 displacing IGF-2 and thus augments its free circulating concentration