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Furthermore, since the derived R h value can be used for the evaluation of the frictional coefficient, f , and the theoretical minimum frictional coefficient f 0 can be calculated for a perfect, unhydrated/unsolvated sphere with a radius of R u and a volume equivalent to that of the macromolecule being studied, one can obtain information about the frictional ratio, f/f 0 = R S /R u , which is independent of molecular mass and provides information on the shape of a studied protein, being equal to unity for a spherical unsolvated protein

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