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Laccase activities were measured using 190 l of reaction mixtures (100 mM citratephosphate buffer pH 4.0 and 1 mM ABTS) in the presence and the absence of a gradient of NaCl (5190 mM) and 10 l of purified LacAnc100 (0.34 g ml 1 ) or LaccID (0.40 g ml 1 ) in flat-bottom 96-well plates (Greiner Bio-One, 655101)

We did not observe any gross toxicity in normal mice with the doses used in this study

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Using this tool, many new non-histone lactylation sites were identified, which expanded the known substrate scope of protein lactylation (Sun et al., 2022)