Paraffin blocks were sectioned onto SuperFrost Plus slides, dried overnight, and baked for 1 h at 60 C before immediately proceeding with the RNAScope Multiplex Fluorescent v2 assay according to the manufacturers instructions with the following modifications: Target retrieval was performed for 15 min in a boiling beaker, Protease III was used for 30 min pre-treatment at 40 C and hybridized slides were left overnight in 5 SSC before proceeding with the amplification and labeling steps
Olsson CA, Coffey C, Toumbourou JW, Bond L, Thomas L, Patton G (2003) Family risk factors for cannabis use: a population-based survey of Australian secondary school students
Intricacy of the Seals The cylinder seal came into popular use during the fourth millennium BCE in the Middle and Late Uruk Period
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God forbids it in the First Commandment: You shall have no other gods before Me (Ex
