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Western blotting The protein extracts of the lung tissues and cells were prepared using Radio Immunoprecipitation Assay (RIPA) buffer (Phygene, China) with Phenylmethanesulfonyl fluoride (PMSF) (Solarbio, China), Next, the protein concentrations were tested by a protein quantification kit (BCA Assay) (Zomanbio, China)

How to avoid secondhand smoke while living with a smoker The best way to avoid secondhand smoke is to not allow smoking in your home

Humidifying stones or packets (e.g., Boveda packs) can help maintain the ideal 1215% range

So what does this have to do with smoking

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