TGR inhibitors do not react covalently with GSH, selenocysteine, or TGR, and inhibition of TGR is reversible To evaluate stability of the TGR inhibitors in the presence of GSH and selenocysteine, compound 2 and a known covalent inhibitor of TGR, TRi-1 20,45 , were incubated with GSH or the N-Boc protected methyl ester of selenocysteine, and the reaction mixtures were analyzed by LCMS (Supplementary Figs
On the basis of such standard assays, different isoforms are hereinafter referred to as enzymatically active or inactive glutaredoxins for the sake of simplicity (without excluding the possibility that inactive isoforms might actually catalyse other reactions with specialized substrates in vivo )
Tu et al , 2000
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Antiarthritic effect The animal-related experiment procedures in this work were conducted according to the guidelines approved by the Animal Ethics Committee of Shanghai Tenth Peoples Hospital, Tongji University School of Medicine (SHDSYY-2020-Z0026/1)
