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[27] A 2015 review concluded that "Nicotine acts as a gateway drug on the brain, and this effect is likely to occur whether the exposure is from smoking tobacco, passive tobacco smoke or e-cigarettes." [28] Nicotine may have a profound impact on sleep

The trouble with this study is that it was done at a very high voltage level

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The effect of CSE treatment on epithelial cells was assessed with the human alveolar basal epithelial cell line A549, the human nasal epithelial cell line RPMI2650, the human bronchial epithelial cell line BEAS-2B, or the mouse lung epithelial cell line MLE12

Criteria for a recommended standard: occupational exposure to diacetyl and 2,3-Pentanedione