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Sclerostin [a sclerosteosis ( SOST ) gene product] is an essential component of the negative regulator in bone development, and osteocytes and osteoblasts may play a major role

Vacuum erection devices revisited: its emerging role in the treatment of erectile dysfunction and early penile rehabilitation following prostate cancer therapy

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After 24 h, medium was replaced, and cells were transfected with 500 ng of the respective pcDNA5 vector using 2 l of Lipofectamine 2000/12-well (Thermo Fisher Scientific) in OptiMEM (Thermo Fisher Scientific)