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When used alone, the inhibition of 5-LOX by Zileuton led to the accumulation of AA

To identify the specific mechanisms by which cystine affects bacterial cells, we measured the GSH/GSSG ratios, intracellular ferrous iron concentrations, and ROS levels in bacteria cultured with exogenous cystine

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For MS/MS, the following settings are used: isolation window width 1.2 m/z with an offset of 0.5 m/z, stepped NCE at 10, 15, and 25 a.u., minimum AGC 5E 2 , and dynamic exclusion of previously sampled peaks for 8 s

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