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Ledford, D

Immunoprecipitation assay To check protein-protein interaction, HeLa cells were lysed using cell lysis buffer (20 mM TrisHCl pH 7.5, 1 mM EDTA, 150 mM NaCl, 1 mM EGTA, 1% Triton X100, 1 mM sodium orthovanadate, 2.5 mM sodium pyrophosphate, 1 mM PMSF and 1 protease inhibitor cocktail)

The regeneration of GSH after an APAP dose is also affected by the concentration of glutamate in the liver and it is known that supplementation of rats with glutamine prior to and APAP over dose acts to preserve GSH levels