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You can read more about this here: A review of adverse effects from IV glutathione

Effect on cell viability was assessed 72 h after transfection with GSTO1 siRNA or siSCRAM by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and normalized to mock-transfected control (transfection reagent)

Energetically favorable literally means that it has a more negative Gibbs free energy, which typically means that the protein is more compact and makes more interactions with itself, like in the rough sketch of a protein on the right side of Figure 6

J.WilliamsD

Cell debris was removed by centrifugation at 4,400 g at 4 C for 30 min

D.EdwardsR