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the plasma was stored at 80 C until assays were performed

To neutralize ROS, cells induce various antioxidant systems, including the activation of the protein machinery that produces glutathione [10], the most abundant antioxidant scavenger in all cells [11, 12]

T.LangW.et al (2000)

Submicromolar concentrations of 4-hydroxynonenal induce glutamate cysteine ligase expression in HBE1 cells

Intracellular ROS measurement LNCaP cells were seeded at 6 10 4 cells/well in 24-well plates, adhered overnight and then incubated with conditioned media for 2.5 days before treatment with doxorubicin or taxol

E., Liu, Y., Dopp, I