the fat layer is consistent, accessible, and easy to pinch Anterior thigh practical for self-injection, though typically more sensitive than the abdomen Outer upper arm (lateral deltoid fat) requires a second hand or assistance
3.0 mL Syringe: 300 units max ideal for larger intramuscular injections for NAD+, Glutathione, and other support compounds
All mice received 2 days of 10 mL/kg body weight sterile saline subcutaneous injections before beginning the treatment phase to allow for acclimation to the handling and stress associated with injections
doi: 10.1016/s0306-9877(03)00227-5
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