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In brief, HUVECs were lysed in Co-IP buffer containing 20 mM Tris-HCl (pH 7.5), 100 mM NaCl, 0.5 mM EDTA, 0.5% NP-40, 0.5 mM PMSF, and 0.5% protease inhibitor cocktail on ice for 20 min

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The molecule has become the canonical chemical probe for the entire NNMTmethylationNAD axis and is the foundation for a growing field of methylation-rejuvenation research