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Ice-cold MeOH (600 L), CHCl 3 (200 L), and H 2 O (500 L) samples were vortexed and then centrifuged (10,000 g , 10 min, 4 C) to precipitate proteins

Mechanism of hyperammonemia in patients with GDH-HI In hepatocytes, elevated GDH activity leads to an increased rate of glutamate decomposition to ammonia

The left ureter was exposed via a left flank incision, ligated with 4-0 silk at two points, and cut between the two ligation points

They also assessed gut microbial composition by culturing fecal samples on selective media

In some embodiments, the otic formulation or composition comprises about 16% by weight of the viscosity modulating agent(s)