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Samples were homogenised in NP40 lysis buffer using the Precellys tissue homogeniser, centrifuge, and analysed by western blotting

In DM-II, long-term co-administration of Met and LHR agonists increases the steroidogenic action of 5-amino-N-tertbutyl-2(methylsulfanyl)-4-(3(nicotinamido)phenyl)thieno[2,3-d] pyrimidine-6 carboxamide (TP03) and gonadotropin []

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Lipotropic agents, particularly choline, have been shown to reduce liver fat accumulation, which is a key factor in metabolic health