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Co-culture of HUVECs and THP-1 cells When the HUVEC confluency met approximately 6070%, the conditioned medium collected from THP-1 cell supernatants stimulated by hypoxia intervention and ECM was added into 6-well plates at a ratio of 1:1 and incubated for 24 h to simulate cell co-culture
The addition of ascorbic acid could be considered as a strategy for preventing folate degradation during processing 193,346,365,371,430,431,457,475,477,544,545,546,547,548,549
L-carnitine appears to increase oxidation processes which enhance oxygen and blood flow
May support collagen and elastin production in skin