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McLaren AMR, Kawaja MD

10.1007/s00134-007-0843-4 49 Medvidovic-GrubisicM.StambolijaV.KolencD.KatancicJ.MurselovicT.Plestina-BorjanI.et al (2017)

For iHLO differentiation, iPSCs were dissociated with Versene (Thermo Fisher Scientific, catalog: 15040066) on Day 0 and plated into an AggreWell plate (STEMCELL Technologies, catalog: #34421) in Stemflex Medium (Thermo Fisher Scientific) to form aggregates, which were then transferred to an ultralow attachment dish on Day 1 and cultured in RPMI1640 supplemented with B27 supplement minus insulin (RPMI/B27-Insulin), CHIR99021 (1 M), Activin A (100 ng/mL), WNT3a (50 ng/mL), and Rock Inhibitor (Y27632, 5 M)

BPC-157 as an antidote to local anesthetics Presenting BPC-157 peptide as an antidote, using lidocaine as an example, allows classification of its action profile leading to depolarization in non-transfected HEK 293 cells

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Decreased appetite and reduced food intake