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Statistical analysis was performed using GraphPad Prism 8.0 (GraphPad Software, San Diego, CA, USA)

Long-term storage (months to years) : Store at -80C (ultra-low temperature freezer)

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However, the extent of ROS generation significantly differed that posttreatment inhibited the ROS generation by 58.4%, whereas pretreatment suppressed the generation of ROS by 90.5%

Then, the immunoprecipitated DNA fragments were extracted with Min-Elute PCR purification kit (Qiagen, 28004)