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All plasmids were verified by DNA sequencing (COME company, Jilin, China)

97 Park et al showed that the antiproliferative effect of delphinidin was achieved through the regulation of the PI3K/Akt and MAPK signaling pathways

It is well worth having someone else check your new calculation, as the last thing you want is a compounded error

doi: 10.1038/s41598-018-21421-x 235

Astrocyte-specific or enriched ncRNAs act as finely tuned molecular switches that determine astrocyte phenotypes and regulated process such as NF-B signaling, NLRP3 inflammasome activation and synaptic homeostasis

Percentage of correct classification was 62.5% with Nagelkerke r 2 = 0.079 (Table 2)