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The expression of specific mRNAs was assayed by fluorescence-based quantitative real-time PCR (RT-qPCR) using a Fast-SYBR PCR Master Mix (Applied Biosystems)

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Gelders, G., Baekelandt, V

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Measurement and characterization of the denitrosation of tauromustine and related nitrosoureas by glutathione transferases in liver cytosol from various species