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For CYP3A4 inhibition testing, ketoconazole (MilliporeSigma, catalog: PHR1385) was prepared at 2 final concentration in culture medium and added in equal volume to each well of a 384-well plate for 24 h

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L-Carnitine forms are usually higher-dose materials, and many are hygroscopic, which can reduce flowability, increase clumping risk, and create capsule-weight variation under poor humidity control

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Target cells were labeled with 0.5 g/mL pHrodo dye (ThermoFisher) for 1 h at room temperature