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Further, we did not detect a correlation of GSH status and levels of MDA or total cysteine, suggesting that the GSH buffer capacity is not a limiting factor for protection from oxidative stress in this cohort of healthy older adults

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When the temperature was lowered to 20 C, the mixture was centrifuged at 10,000 g for 20 min, after which absorbance values were measured at 600, 450, and 532 nm

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