doi: 10.1038/ng.2982 18
Lineage-negative cells were enriched using the lineage cell depletion kit (Miltenyi Biotec, 130-110-470), differentiated in the presence of 20 ng ml 1 M-CSF (PeproTech, 315-02) for 3 days, treated with 10 g ml 1 -glucan or PBS for 24 h, cultured in the presence of M-CSF for 3 days, induced with 10 ng ml 1 LPS for 24 h before the medium was collected for quantification of cytokines
Competitive analyte elution is much more specific for the bait-prey interaction, because it does not strip proteins that are nonspecifically bound to the affinity support
[DOI] [PubMed] [Google Scholar] 69.Olanow CW, Goetz CG, Kordower JH, et al
Sprawia, e cera staje odpowiednio nawilona, gadka i promienna
Active joint infection represents an absolute contraindication to intra-articular injection of any substance, including AOD-9604