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coli BL21(DE3) containing plasmid pANT43 was inoculated from an overnight preculture at a 1:100 ratio in four 2 l baffled Erlenmeyer flasks filled with 400 ml TB medium containing 50 g ml 1 kanamycin sulfate and incubated at 37 C, 140 rpm using a 5 cm shaking diameter for 2.75 h

Since the cytosol contains high concentrations of glutathione and is, therefore, a reducing environment, proteins containing disulfide bonds, which are formed from oxidized cysteine residues, cannot be produced within it

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A full list of survey questions can be found in Supplementary Table 24