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Reverse-phase HPLC purification

Initially, these phagocytes adopt a pro-inflammatory, microbicidal program characterized by elevated production of reactive oxygen species (ROS), nitric oxide (NO), proteolytic enzymes, and pro-inflammatory cytokines like IL-1, IL-6, and TNF-, enabling efficient pathogen clearance [39]

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The literature screening process was independently completed by two researchers, with disagreements resolved through discussion or consultation with a third researcher