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Hirano M, Matsuki T, Tanishima K, et al

To the 10.000 rpm supernatant containing microsomes and cytosol fractions, 0.2 volume of 0.1 M calcium chloride solution was added, and further centrifuged at 10,000 rpm for 10 minutes to precipitate the microsomes

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& Badry, R

Kong L, Deng J, Zhou X, Cai B, Zhang B, Chen X, Chen Z, Wang W

(2017), Clinical, Cosmetic and Investigational Dermatology , reviewed both topical and oral glutathione studies for skin lightening, concluding that effects are generally modest and reversible upon discontinuation