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Increased -SMA expression in primary lung fibroblasts from aged (24 months) C57BL/6 mice compared to young (8 months) C57BL/6 mice

GI events (nausea, vomiting, diarrhea) were the dominant safety signal and occurred at rates higher than reported for Phase 2 retatrutide and higher than for comparable dual or mono-agonist trials

The increase in GH secretion precedes the reduction in IGF-I levels that is only evident by the third day of fasting, thereby ruling out the increase in GH during fasting is related to reductions in total IGF-I

the only cross-drug data are weak indirect network meta-analyses

In cognitive peptides: substitute when possible in vivo tests by cerebral organoids or primary neuronal cultures ( Replacement ), optimise experimental plan to minimise the number of animals ( Reduction ), use appropriate anaesthesia and analgesia during invasive procedures ( Refinement )

J Mammary Gland Biol Neoplasia