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This is part of why it's studied for its effects on amphetamine-induced behaviors and as a potential neuroprotective agent

Vitamin B12 deficiency can lead to the development of megaloblastic anemia, demyelination, and other types of neurological damage

From a physiological point of view, the paracellular route and the transcellular endocytic route are the two relevant pathways in the context of the leaky gut

doi: 10.1016/j.cellsig.2013.06.007 317 MattagajasinghIKimCSNaqviAYamamoriTHoffmanTAJungSBet al

There is also a practical reason

For any experimental design or health-related decision , always consult qualified professionals and institutional guidelines