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Briefly, custom crRNAs were complexed with trRNAs to form 1 M gRNAs

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Beads were then washed 2x in batch format with wash buffer 1 (50 mM Tris pH 7.4, 150 mM NaCl, 10 mM EDTA, 1 mM MgCl 2, 0.05% NP40) and then 3x with wash buffer 2 (same as wash buffer 1 but without detergent)

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