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doi: 10.1097/ACI.0B013E3282F3D913 55 Bankers-FulbrightJLKitaHGleichGJOGradySM

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Altobelli E, Del Negro V, Angeletti PM, Latella G

Some reasons your body may not be able to get enough B12 from food include: Anemia Gastritis Crohns disease Celiac disease Alcohol use disorder Sjgrens syndrome Weight loss surgery, or surgery that removes part or all of your stomach A vegan or strict vegetarian diet (as B12 is only found in animal products) Medications, such as metformin and drugs used to treat gastroesophageal reflux disease (GERD), indigestion, and stomach ulcers Pregnancy Your doctor could also order a B12 test based on your symptoms

In some casesparticularly when performing on-column tag cleavagea larger buffer volume is required to elute the tagged protein