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Less movement during the day

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After blocking with 5% bovine serum albumin (BSA, Sigma-Aldrich) in TBST (10 mM Tris-HCl, 150 mM NaCl, and 0.1% Tween 20) for 1 h at room temperature, the membrane was incubated with primary antibody overnight at 4 C

Lu B, Lu Y, Moser AH et al (2008) LPS and proinflammatory cytokines decrease lipin-1 in mouse adipose tissue and 3T3-L1 adipocytes

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The NICE recommendations tell GPs to consider testing in anyone with one symptom or one risk factor, and to treat early to prevent neurological damage that can become permanent