GSH assay The cells were incubated with ACE, erastin, and RSL3 for 24 h
GPX deficiency leads to an accumulation of H 2 O 2 and leaves cells vulnerable to oxidative stress [56]
Doctors can also utilize a classic surgery called an Ileal Pouch-Anal anastomosis
All cell lines except hTERT RPE-1 cells were cultured in DMEM (4.5 g l 1 glucose, Thermo Fisher Scientific) supplemented with 10% (v/v) FBS (Thermo Fisher Scientific), 100 U ml 1 penicillin (Thermo Fisher Scientific) and 100 g ml 1 streptomycin (Thermo Fisher Scientific)
Moreover, the increased production of RBC is associated with higher requirements for folate, vitamin B 12 , vitamin B 6 and riboflavin due to their involvement either in the generation of new cells or in the synthesis of Hb, which in turn would further compromise the status of these B-vitamins
Montopoli M, Bellanda M, Lonardoni F, Ragazzi E, Dorigo P, Froldi G, et al