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Cadmium induces multi-organ toxicity, primarily targeting the liver by disrupting hepatocyte function and activating cell death pathways

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This duration of safety monitoring provides meaningful reassurance about sustained exposure effects that would not be apparent in brief trials

The % of cytotoxicity was measured using the following formula: Click-iT plus TUNEL assay To perform TUNEL Alexa Fluor Staining, the commercial Click-iT TUNEL Alexa Fluor Imaging Assay (C10247, Invitrogen , Warsaw, Poland) was used in accordance with the instructions provided by the manufacturer

However, in contrast to the aforementioned studies, Chanez and colleagues found decreased expression of NPY in the airway smooth muscle, but not in the epithelium, of people with asthma [180]