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J Biol Chem 278:4686946877 Conklin PL, Foyer CH, Hancock RD, Ishikawa T, Smirnoff N (2024) Ascorbate acid metabolism and functions

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Unlike single-pathway therapies, Tirzepatide is a dual-action medication that activates both GLP-1 and GIP receptors simultaneously addressing appetite, satiety, insulin response, and fat metabolism

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For conditional MOR knockdown in PVN Glu neurons, we injected the rAAV-CMV-DIO-(EGFP-U6)-shRNA ( Oprm1 )-WPRE-hGH pA (AAV-DIO-shMOR-EGFP) virus or rAAV-CMV-DIO-(EGFP-U6)-shRNA(scramble)-WPRE-hGH polyA (AAV-DIO-EGFP) vector control into the bilateral PVN of VgluT2-Cre mice