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Samples were vortexed and centrifuged at 21,000 g for 10 min at 4 C

Ceramides are intercellular lipids naturally found in our skin that bonds dead skin cells together to create a barrier

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Statistical analyses To explore differences in cortisol concentrations between the control and the test day after the arrival of the zoo vet, we used linear mixed models (LMM) using the lmer function from the lme4 package 53

An acetylation/deacetylation cycle controls the export of sterols and steroids from S

Afterward, the plates were washed three times with sterile PBS to remove any unbound collagen