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Cells were seeded in 8-well LabTek removable chamber slides (Thermo Scientific, 177402) to 60%70% of confluence

Failing to protect the peptide from prolonged exposure to extreme heat can accelerate degradation, reducing the efficacy and purity of the compound before it even reaches the laboratory workbench

Because these are time-dependent events, it is critical to always assess multiple time points in a pathophysiology and use parameters specific for apoptosis, while keeping in mind that many apoptosis parameters can also be modulated during a necrotic cell death

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