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An increasing number of clinical and laboratory studies have revealed the pathophysiological changes of DR [158]

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For the assembly of the DDA1-DET1-DDB1-Ube2e2-COP1-c-Jun complex, untagged DDB1, TSF-tagged human DET1, N-terminal GST- and C-terminal His-tagged COP1, untagged DDA1, MBP-tagged Ube2e2 S76G-C139S and MBP-tagged c-Jun were co-transfected into Expi293F cells as previously described

Sirtuins, also known as NAD + -dependent class III HDACs, remove the acetyl groups from histone, which restores the electrostatic affinity between DNA and histones to stabilize the chromatin architecture

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