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Attack on DNA may trigger cell apoptosis or gene mutation, affect the normal proliferation and differentiation of thyroid cells, further damage the synthesis and secretion of thyroid hormone, and eventually aggravate the hypothyroidism condition (Lushchak, 2014)

Stenson, B

Geroprotective effect of Epithalamine (pineal gland peptide preparation) in elderly subjects with accelerated aging

Endocrinol Res Pract

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The cells were collected by centrifugation at 2000 g for 5 min and washed in phosphate buffered saline (8 gL 1 NaCl, 0.2 gL 1 KCl, 1.44 gL 1 Na 2 PO 4 , 0.24 g L 1 KH 2 PO 4 , at pH 7.5)